Evosep at US HUPO 2025

We are thrilled to once again participate at the annual Human Proteomics Conference, which will take place in Philadelphia, PA on February 22 – 26, 2025. Please visit us at our booth #6 , where will be happy to introduce you to our technology and how we can contribute with fully automated and standardized workflows. You are also welcome to join our Evosep Breakfast Seminar and/or our User Appreciation Event, register and secure your seat now.

Scientific presence

Our booth is not the only place to learn more about Evosep and the many research possibilities available with the Evosep One. We are excited to share several brand-new insights during US HUPO 2025

poster

Proof-of-concept study: A cost-efficient and scalable plasma proteomics pipeline utilizing the Evotip Pure subjected to more than 1,100 patient samples

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Monday, February 24th, 2025: 07:15AM – 08:15AM – Room: Commonwealth D.

US HUPO 2025 breakfast seminar

STANDARDIZING AND SCALING AUTOMATED WORKFLOWS FOR CUTTING EDGE PROTEOMICS

Join us for breakfast and get the newest data of proteomics workflows, scientific discussions, and new discoveries from Evosep. Learn more about the full programme at US HUPO 2025

Monday, February 24th, 2025: 07:15AM – 08:15AM – Room: Commonwealth D.

US HUPO 2025 breakfast seminar

STANDARDIZING AND SCALING AUTOMATED WORKFLOWS FOR CUTTING EDGE PROTEOMICS

Join us for breakfast and get the newest data of proteomics workflows, scientific discussions, and new discoveries from Evosep. Learn more about the full programme at US HUPO 2025

Introducing SysQuan: Affordable Single-Injection Absolute Quantitative Proteomics using Stable Isotope-Labeled Mouse

Talk by Christoph Borchers, Scientific Advisor at MRM Proteomics

Large-scale proteomics faces challenges like batch effects, long LC run times, and high costs of SIL standards. Our innovative workflow SysQuan, using SIL mouse as an internal standard, enables absolute human proteome quantitation, simplifying workflows and reducing costs. Human and mouse liver homogenates and plasma were digested and analyzed in a one-to-one mix, resulting in over 6,900 human liver and 1,400 plasma proteins in untargeted DDA-PASEF using the Evosep One coupled to a Bruker timsTOF HT. MRM quantified up to 300 hepatic proteins within a single injection, demonstrating the high performance of the Evosep combined with the Agilent 6495D.

**Sequencing antibodies from de novo to identify functional and neutralizing antibodies in human plasma after SARS-CoV-2 vaccination**

Talk by Thierry Le Bihan, Principal Scientist at Rapid Novor

While B-cell sequencing has dominated SARS-CoV antibody discovery (accounting for 90% of findings in the CoV-AbDab database), this approach has limitations. It often fails to connect B-cells with circulating IgG antibodies, which are the primary agents of humoral immunity. Most IgGs are produced by bone marrow plasma cells rather than circulating B-cells. Although combining proteomics with B-cell repertoire analysis can confirm the presence of specific antibodies in circulation, it cannot identify antibodies missed by B-cell sequencing. De novo pAb sequencing, despite its technical challenges, offers a promising method to better understand the complete humoral immune response.

Monday, February 24th, 2025: 08:00PM – 11:00PM – Iron Hill brewery

Evosep User appreciation Event

Join us Monday, February 24th at our User Meeting at US HUPO 2025 and meet company experts as well as fellow Evosep One users for informal networking, buffet with refreshments, and knowledge exchange.

Location of the event will be at Iron Hill Brewery – City Center, 1150 Market St, Philadelphia, PA 19107.

The distance to the event from the Philadelphia Loews Hotel is 300 feet, which takes approximately 1 minutes by foot. The Iron Hill Brewery is located next door from the hotel.