Immunopeptidomics
Immunopeptidomics is an emerging field that combines proteomics and immunology to study MHC-bound peptides.
Discover how Evosep One accelerates discoveries, and support the understanding of immune responses and diseases.
Immunopeptidomics
Immunopeptidomics is an emerging field that combines proteomics and immunology to study MHC-bound peptides.
Discover how Evosep One accelerates discoveries, and support the understanding of immune responses and diseases.
Recent Technological advancements have evolved the fieldÂ
Immunopeptidomics is a rapidly evolving field at the intersection of proteomics and immunology, aiming to unravel the complex landscape of peptides presented by major histocompatibility complex (MHC) molecules on the cell surface.
In recent years, technological advancements have revolutionized the field of immunopeptidomics, enabling high-throughput analysis of MHC-bound peptides. Innovations in mass spectrometry, liquid chromatography, and sample preparation have significantly increased sensitivity and throughput, allowing for the comprehensive characterization of immunopeptidomes.
Live webinar
Advancing Immunopeptidomics with Sensitivity, Robustness and High-Throughput
April 24th 2025 at 4:00 PM CET
In this webinar, we explore how Evosep One is transforming immunopeptidomics by enabling faster, more reproducible, and high-throughput workflows, helping researchers unravel immune responses with greater precision.
transition From specialized application to general applied field with Evosep One
Immunopeptidomics as a field has moved from the domain of just a handful of specialised laboratories to now being more generally applied by the proteomics and immunology communities.
The Evosep One combines high-performance liquid chromatography (HPLC) with integrated sample handling, delivering robust and reproducible separations with minimal sample loss. Its unique capabilities make it particularly well-suited for immunopeptidomics studies, enabling researchers to analyze large sample cohorts efficiently.
This advancement has transformed immunopeptidomics into a versatile tool, fueling breakthroughs across immunity research, cancer studies, and disease biomarker identification, impacting diverse fields beyond its specialized origins.
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HELMHOLTZ INSTITUTE FOR TRANSLATIONAL ONCOLOGY MAINZ (HI-TRON MAINZ)
MAETi: MILD ACID ELUTION IN A TIP ENABLES QUANTITATIVE IMMUNOPEPTIDOME PROFILING FROM 25,000 CELLS
This study from HI-TRON Mainz introduces MAETi, a novel workflow for high-sensitivity MHC-I immunopeptidome profiling.
Using the Evosep One with the Whisper 40 method and Evotip, researchers achieved efficient isolation and analysis of HLA-bound peptides from as few as 25,000 cells. The Evotip played a crucial role in streamlining sample preparation, resulting in cleaner samples and enhanced immunopeptidome coverage.
Key results include a significant reduction in total analysis time and high quantitative accuracy, making the MAETi workflow, combined with the advanced capabilities of the Evosep One, a valuable tool for scalable immunopeptidomics research. This integration enabled comprehensive profiling from a wide range of cell counts, paving the way for more efficient and reproducible studies.
Read full publication here
SHIRAZ UNIVERSITY OF MEDICAL SCIENCES
DISCOVERY OF NOVEL AUTOANTIBODIES IN UROTHELIAL BLADDER CANCER USING LCMS
The identification of novel autoantibodies holds significant importance in advancing the diagnosis and treatment of urothelial bladder cancer (UBC). In a recent publication from Shiraz University of Medical Sciences, researchers utilized an immunoproteomics approach to discover new tumor-associated antigens (TAAs) in UBC patients.
The study employed the Evosep One with the Whisper 40 method and Evotip to efficiently isolate and analyze TAAs from patient samples. This advanced setup significantly enhanced the detection and quantification of specific proteins, leading to the identification of eight novel anti-TAA autoantibodies. The samples were analyzed using the 100 SPD method.
This integration of the Evosep One streamlined the sample preparation process, resulting in cleaner samples and improved immunoproteome coverage. The study highlights the potential of these autoantibodies as diagnostic and prognostic biomarkers, paving the way for better clinical management of UBC.
Read full publication here
Biomedicine Discovery Institute, Monash University
Multiple Classes of Antigen Contribute to the Antigenic Landscape of Mesothelioma
Researchers conducted a detailed study on the human leukocyte antigen (HLA) class I landscape of mesothelioma using patient-derived cell lines and pleural effusion samples.
They identified over 2000 peptides, including those from cancer testis antigens (CTAs), endogenous retroviruses (ERVs), and tumor-associated antigens (TAAs). A unique cysteinylation signature was also observed in post-translationally modified peptides.
Using advanced liquid chromatography-mass spectrometry (LC-MS) systems, including the Evosep One, the team efficiently analyzed HLA-bound peptides. These findings provide important insights into the diverse antigenic landscape of mesothelioma, which could help in future vaccine studies and immunotherapy approaches.
Read full publication here
EVOSEP WEBINAR
explore recent advancements, challenges, and applications in immunopeptidomics
Webinar
Advancing Immunopeptidomics: Leveraging Evosep One for High-Throughput Analysis and Discovery
Available on demand
Join our webinar to explore recent advancements, challenges, and applications in immunopeptidomics. Discover how Evosep One accelerates discoveries, and support the understanding of immune responses and diseases.
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Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth
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end-to-end, fully automated digestion protocol and evotip pure workflow on the opentrons ot-2
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fully automated, rapid, robust sample loading on evotip pure with the agilent assaymap bravo
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