Drug Discovery
As proteins are the targets for most drugs, proteomics is important to study novel targets and understand disease mechanisms and toxicity.
Explore, how the Evosep One technology is a valuable tool for high-throughput and reliable drug discovery.
Streamlining drug discovery and development
Proteomics is transforming drug development, from early research to clinical application. In discovery, it enables deep proteome insights for drug target identification and validation.
As candidates progress, proteomics aids in lead optimization, toxicity profiling, and drug response analysis. In preclinical and clinical phases, it ensures drug safety, efficacy, and biomarker identification for precision medicine.
Standardization is key for assays transfers throughout this journey, delivering scalable, high-performance LC-MS based proteomics – from deep discovery analysis to high-throughput clinical studies. Its seamless integration of sample preparation and LC-MS ensures efficient, reliable, and cost-effective proteomics at every stage.
High-throughput drug discovery with the Evosep OneÂ
Drug discovery is the process through which potential new medicines are identified. It involves a wide range of scientific disciplines, including biology, chemistry and pharmacology.
The complexity in drug development has increased over the past 40 years, requiring preclinical testing, investigational new drug applications, and completed clinical testing before marketing approval from the FDA.
The overarching goal is to bring more efficient and safer treatments to the patients as quickly as possible after a thorough medical evaluation.
High-throughput and sensitive analysis on the Evosep One is a good fit when developing new drug applications or biologics license applications.
Live webinar  I  March 27th 2025 at 4:00 pm CET
Industrialized drug discovery with the Evosep One
Join this webinar to learn how industrialized proteomics combined with the Evosep One can enhance drug discovery pipelines, reduce bottlenecks, and bring safer treatments to patients faster.
Novo Nordisk Foundation Center for Protein Research, University of Copenhagen, Copenhagen
Enhancing Drug Target Identification with Evosep One
Published in Nature Communications, this study from the Olsen group highlights the potential of using the Evosep One in optimizing Thermal Proteome Profiling (TPP) and PISA assays for drug target identification.
Traditional workflows can be time-consuming and prone to variability, limiting large-scale studies. By integrating direct Evotip loading and reducing manual handling steps, Evosep One streamlines sample preparation, increases throughput, and improves data quality.
Combined with DIA-MS analysis using DIA-NN software, this approach enhances reproducibility and enables high-throughput proteomics, making it a valuable tool for drug discovery research.
Read full publication here
Barnett Institute of Chemical and Biological Analysis & Northeastern University
Improving Drug Discovery with New Techniques
In the pursuit of more effective drug discovery, mass spectrometry-based thermal stability assays (MS-TSA) have emerged as a promising solution for identifying protein-ligand interactions.
This study published in Nature explores innovative strategies to improve drug-target identification using MS-TSA. The study investigates eight combinations of advanced MS-based techniques, including Phase-Constrained Spectral Deconvolution Method and Field Asymmetric Ion Mobility Spectrometry, to enhance the performance of thermal stability assays.
By treating intact Jurkat cells with a commercially available MEK inhibitor and subjecting them to heat treatment, the researchers demonstrated significant improvements in protein identifications and high-quality melting curves compared to conventional approaches.Â
Abbvie
Automated High-throughput Affinity Capture-Mass Spectrometry with Evosep One
This work showcases an automated high-throughput affinity capture-mass spectrometry (AC-MS) workflow using the Evosep One to enhance drug discovery.
Researchers used biotinylated probes and streptavidin magnetic beads to enrich small molecule targets in a 96-well plate format. Samples were then directly taken from Evotips for LC-MS/MS analysis. This streamlined workflow significantly reduced both overall and hands-on time for sample preparation.
The study also introduced a data-independent acquisition-mass spectrometry (DIA-MS) method, covering approximately 380 kinases—over 60% more than traditional methods.
Read full publication here
Pelago biosciences
Robust and Reproducible Protein Quantification in PlasmaÂ
At Pelago Biosciences, they have stablished the Cellular Thermal Shift Assay (CETSA) in a high-throughput drug discovery pipeline with the Evosep One, enabling them to analyze a large number of compounds and unravel common or distinct pathways activation.
In this publication, they present a deep and comprehensive profiling of Staurosporine induced proteome thermal stability alteration utilizing different experiment layouts.
Ultimately, they collected data from 14 individual replicates of Staurosporine-treated K562 lysates, which allowed them to quantify more than 7500 proteins.
From this comprehensive dataset, they were able to detect 293 proteins with concentration dependent changes in thermal stability – 169 stabilized proteins (117 kinases – in blue) and 63 (11 kinases – in red) destabilized proteins.
EVOSEP WEBINAR
MEET OUR USERS and their research on drug discovery
High-throughput drug discovery with the Evosep One
Available on demand
In this webinar we will look into how the Evosep One with high-throughput and sensitivity is a good fit when developing new drug applications or biologics license applications.
explore more
Find all publications, product brochures and presentations with Evosep One
DOWNLOAD OUR MATERIAL
application note
Evosep One solves the proteomics dilemma – covering high throughput analysis and proteome depth
application note
end-to-end, fully automated digestion protocol and evotip pure workflow on the opentrons ot-2
application note
USING A COST-EFFICIENT AND SCALABLE PLASMA PROTEOMICS PIPELINE TO ANALYZE MORE THAN 1100 PATIENT SAMPLES
brochure
EVOSEP ONE: A Standardized Separation
Tool for proteomics
Want to know more?
Our team is ready to help you find the method fitting your research need. Fill out our contact form and we will get back to you as soon as possible
